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Everything about Chemical Test totally explained

In chemistry, a chemical test is qualitative or quantitative procedure designed to prove the existence of, or to quantify, a chemical compound or chemical group with the aid of a specific reagent. A presumptive test is specifically used in medical science.

Biochemical tests

Reducing sugars

  • Barfoed's test tests for reducing monosaccharides or disaccharides
  • Benedict's reagent tests for reducing sugars or aldehydes
  • Fehling's solution tests for reducing sugars or aldehydes, similar to Benedict's reagent
  • Molisch's test for carbohydrates

    Proteins and polypeptides

  • The Bicinchoninic acid assay tests for proteins
  • Biuret reagent tests for proteins and polypeptides
  • Bradford protein assay measures protein quantitatively

    Organic tests

  • The Carbylamine reaction tests for primary amines
  • The Griess test tests for organic nitrite compounds
  • The Iodoform reaction tests for the presence of methyl ketones, or compounds which can be oxidized to methyl ketones
  • The Schiff test detects aldehydes
  • Tollens' reagent (Silver Mirror) tests for aldehydes
  • The Zeisel determination tests for the presence of esters or ethers
  • Lucas' reagent is used to determine mainly between primary, secondary and teriary alcohols.

    Inorganic tests

  • Barium chloride tests for sulfates
  • The Beilstein test tests for halides qualitatively
  • Borax bead test tests for certain metals
  • The Carius halogen method measures halides quantitatively
  • Chemical test for cyanide tests for the presence of cyanide, CN-
  • Copper sulfate tests for presence of water
  • Flame tests test for metals
  • The Gilman test tests for the presence of a Grignard reagent
  • The Kjeldahl method quantitatively determines the presence of nitrogen
  • Nessler's reagent tests for the presence of ammonia
  • Ninhydrin tests for ammonia or primary amines
  • The sodium fusion test tests for the presence of nitrogen, sulfur, and halides in a sample
  • The Zerewitinoff determination tests for any acidic hydrogenFurther Information

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